hTROP2/MC-38
CBPG0029
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產(chǎn)品描述
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| I. Background | |||
| TROP2 由 TACSTD2 基因編碼，是一種鈣信號(hào)跨膜蛋白，TROP2 在胚胎發(fā)育過(guò)程中以 及惡性腫瘤細(xì)胞的增殖、侵襲、轉(zhuǎn)移具有重要意義。TROP2 在很多健康的器官中表達(dá)，包 括呼吸道、宮頸、子宮內(nèi)膜、結(jié)腸直腸和膽管上皮的肝臟等發(fā)育正常成熟的器官外，胚胎 和胎兒發(fā)育過(guò)程中 TROP2 也會(huì)在肺、腸、胃、膀胱和腎臟中表達(dá)。TROP2 在各種上皮性 腫瘤中特異表達(dá)，通過(guò)增加細(xì)胞中周期蛋白 D1 和 E 的 量來(lái)加速細(xì)胞周期過(guò)程，從而導(dǎo)致 腫瘤的生長(zhǎng)，明顯提高腫瘤細(xì)胞的侵襲力和轉(zhuǎn)移潛能。TROP2 在胰腺癌、乳腺癌、結(jié)腸癌、 胃癌、口腔鱗癌等多種上皮起源的實(shí)體腫瘤中高表達(dá)，在男性前列腺癌中和女性卵巢癌、 宮頸癌等生殖系統(tǒng)惡性腫瘤也高表達(dá)。TROP2 的高表達(dá)也存在于非上皮起源的腫瘤中，例 如黑色素瘤、結(jié)外 NK/T 細(xì)胞淋巴瘤、膠質(zhì)瘤和膠質(zhì)母細(xì)胞瘤以及骨肉瘤。TROP2 在垂體 腺瘤中也被發(fā)現(xiàn)過(guò)表達(dá)。 | |||
| II. Introduction | |||
| Host Cell: | MC-38 | ||
| Expressed gene: | TROP2 | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mus musculus, mouse | ||
| Tissue: | Colon | ||
| Morphology: | Epitheloid cell | ||
| Growth Properties: | Adherent | ||
| IV. Representative Data | |||
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Figure 1. Recombinant hTROP2/MC-38 stably expressing TROP2. |
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| V. In VIVO Data | |||
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Index |
Content |
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Animal |
C57BL/6J, 7weeks, female |
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Implanted Position |
Subcutaneous (right lower flank region ) |
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Injected Cell Numbers |
1×10^6/0.1ml |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hTROP2/MC38 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3. hTROP2/MC38 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
