EGFR Effector Reporter Cell
CBP74269
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| I. Background | |
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EGFR（Epidermal Growth Factor Receptor）是上皮生長因子（EGF）細(xì)胞增殖和信號(hào) 傳導(dǎo)的受體，EGFR 屬于 HER 受體家族，該家族由四種相關(guān)蛋白 EGFR（HER1/ErbB1）、 ERBB2（HER2）、ERBB3（HER3）和 ERBB4（HER4）組成，屬于酪氨酸激酶型受體， 位于細(xì)胞膜表面，靠與配體結(jié)合來激活。EGFR 與腫瘤細(xì)胞的增殖、血管生成、腫瘤侵襲、 轉(zhuǎn)移及細(xì)胞凋亡的抑制有關(guān)。
HER 受體通過與不同的配體結(jié)合而被激活，包括 EGF、TGFA、肝素結(jié)合 EGF 樣生長 因子、雙調(diào)蛋白、β細(xì)胞蛋白和上皮調(diào)節(jié)蛋白。配體與受體的細(xì)胞外結(jié)構(gòu)域結(jié)合后，受體形 成功能活性二聚體[EGFR-EGFR（同型二聚體）或 EGFR-HER2、EGFR-HER3、EGFR- HER4（異二聚體）]。二聚化誘導(dǎo)酪氨酸激酶結(jié)構(gòu)域的激活，從而導(dǎo)致多個(gè)酪氨酸殘基上 的受體自磷酸化。從而導(dǎo)致一系列接頭蛋白（如 SHC、GRB2）的募集，并激活一系列細(xì) 胞內(nèi)信號(hào)級(jí)聯(lián)反應(yīng)以影響基因轉(zhuǎn)錄，進(jìn)而導(dǎo)致癌細(xì)胞增殖、減少細(xì)胞凋亡、侵襲和轉(zhuǎn)移， 并刺激腫瘤誘導(dǎo)的血管生成。 |
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| II. Description | |
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EGFR Effector Reporter Cell 報(bào)告基因藥靶模型很好的模擬了體內(nèi) EGFR 的信號(hào)轉(zhuǎn)導(dǎo)過程， 原理見下圖所示。
 Figure 1. EGFR Effector Reporter Cell 細(xì)胞模型原理圖 |
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| III. Introduction | |
| Expressed gene: | EGFR |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+ 2μg/ml Puromycin+ 200μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
IV. Representative Data Figure 2. Recombinant EGFR Effector Reporter Cell stably expressing EGFR.  Figure 3. Dose Response of Recombinant Human EGF in EGFR Effector Reporter Cell (C9).  Figure 4. Inhibition of rhEGF-induced Reporter Activity by EGFR Blocking Ab in EGFR Effector Reporter Cell (C9). Inhibition of rhEGF-induced Reporter Activity by EGFR Inhibitor in EGFR Effector Reporter Cell (C9). |
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