Integrin avβ6 Effector Reporter Cell
CBP74319
| I. Background | |
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整合素αvβ6 是由αv 和β6 亞基組成的異二聚體跨膜受體，屬于整合素家族成員，其胞 外域通過識別含 RGD（精氨酸-甘氨酸-天冬氨酸）序列的配體介導(dǎo)細胞與細胞外基質(zhì)的相 互作用，主要在上皮細胞中低表達，但在組織損傷、纖維化、炎癥及惡性腫瘤中顯著上調(diào)， 成為疾病治療的潛在靶點。該整合素的主要配體包括細胞外基質(zhì)蛋白如纖維連接蛋白 （Fibronectin）、層粘連蛋白（Laminin）以及潛活形式的 TGF-β，后者通過與αvβ6 結(jié)合被 激活為活性形式，驅(qū)動纖維化和免疫抑制微環(huán)境形成；此外，αvβ6 還可作為口蹄疫病毒 （FMDV）等病原體的入侵受體。 |
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| II. Description | |
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Integrin αvβ6 Effector Reporter Cell 報告基因藥靶模型很好的模擬了體內(nèi)αvβ6 的信號轉(zhuǎn) 導(dǎo)過程，原理見下圖所示。
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| III. Introduction | |
| Expressed gene: | ITGAV、ITGB6 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin+200 μg/ml Hygromycin B+5 μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
IV. Representative Data Figure 2. Recombinant Integrin avβ6 Effector Reporter Cell stably expressing ITGAV、ITGB6.  Figure 3. Dose Response of Recombinant Human Latent TGFβ1 in Integrin avβ6 Effector Reporter Cell (C17).  Figure 4. Inhibition of Human Latent TGFβ1-induced Reporter Activity by Integrin avβ6 Blocking Antibody in Integrin avβ6 Effector Reporter Cell(C17).  |
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Figure 5. Blocking of Human Latent TGF-β1 induced Integrin avβ6 Effector Reporter Cell(C17) Activity by Integrin avβ6 Blocking Ab with GARP/Latent TGF-β1 Target Cell(C5).
Figure 6.Blocking of Human Latent TGF-β1 induced Integrin avβ6 Effector Reporter Cell(C17) Activity by GARP/TGF-β1 Blocking Ab with GARP/Latent TGF-β1 Target Cell(C5).
