Package-Ref? Human Methylation Reference Standard II
CBPE0390
詢 價
索取COA
產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫
| Cat No. | Name | Average Methylation Level | Description |
| CBPE0390-1 | Human Non-Methylation Reference Standard | Average Methy=0% | 本產(chǎn)品通過全基因擴增(WGA)得到，可作為DNA甲基化分析的陰性對照，檢驗方法采用RRBS、ddPCR法和酶切法驗證，酶切法采用McrBc或者MspI和HpaII對樣本進(jìn)行酶切。樣本來源于細(xì)胞系，更接近真實樣本。 |
| CBPE0390-2 | Human Low-Methylation Reference Standard | Average Methy <30% | 產(chǎn)品通過在細(xì)胞層面敲除DNMT3A和DNMT3B蛋白，提取gDNA得到，檢驗方法采用RRBS、WGBS和酶切法驗證，酶切法采用McrBc或者MspI和HpaII對樣本進(jìn)行酶切。樣本來源于細(xì)胞系，更接近真實樣本。 |
| CBPE0390-3 | Human Medium-Methylation Reference Standard | 30% |
本產(chǎn)品通過在細(xì)胞上過表達(dá)DNMT3A和DNMT3B蛋白，提取gDNA得到，檢驗方法采用RRBS、WGBS和酶切法驗證，酶切法采用McrBc或者MspI和HpaII對樣本進(jìn)行酶切。樣本來源于細(xì)胞系，更接近真實樣本。 |
| CBPE0390-4 | Human High-Methylation Reference Standard | Average Methy＞70% |
本產(chǎn)品為天然樣本，檢驗方法采用RRBS、WGBS和酶切法驗證，酶切法采用McrBc或者MspI和HpaII對樣本進(jìn)行酶切。樣本來源于細(xì)胞系，更接近真實樣本。 |
| CBPE0390-5 | Human Hyper-Methylation Reference Standard | Average Methy≈100% | 本產(chǎn)品的全基因組甲基化DNA 是通過甲基轉(zhuǎn)移酶（M.SssI）修飾人的基因組 DNA 而得，可以作為DNA甲基化分析的陽性對照，檢驗方法采用RRBS、ddPCR法和酶切法驗證，酶切法采用McrBc或者MspI和HpaII對樣本進(jìn)行酶切。樣本來源于細(xì)胞系，更接近真實樣本。 |
| Remark | 1. MspI可酶切非甲基化和甲基化的DNA，對甲基化不敏感。 | ||
| 2. HpaII對CpG甲基化敏感，不能酶切甲基化的DNA，只能酶切非甲基化的DNA。 | |||
| 3. 本產(chǎn)品均采用同種細(xì)胞系 | |||
| Representative Data | |||
|
Figure 1. DNA methylation analysis by Reduced representation bisulfite sequencing (RRBS) on different samples.
Figure 2. Different samples digested by MspI or HapII. |
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| Product Information | |||
| Intended Use | Research Use Only | ||
| Concentration | Download for COA | ||
| Purity | Download for COA | ||
| DNA electrophoresis | Download for COA | ||
| Storage | Download for COA | ||
| Expiry | Download for COA | ||
| Remark | The data in the instruction manual is the data of a single test, and the specific data of each batch is subject to delivery. | ||
