hERBB3(HER3)/MC-38
CBPG0036
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產(chǎn)品描述
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| I. Background | |
| ERBB3 又稱 HER3（人表皮生長因子受體 3），是表皮生長因子受體 (EGFR/ERBB) 受體酪氨酸激酶家族的成員。該家族由四個成員組成：EGFR（表皮生長因子受體）、ErbB2 、ErbB3 和 ErbB4 。與 EGFR 不同的是，ErbB2 是一種無配體受體，而 ErbB3 缺乏酪 氨酸激酶活性。因此，ErbB2 和 ErbB3 僅在 ErbB 異二聚體環(huán)境中才有活性，而由 Nrg（神 經(jīng)調(diào)節(jié)蛋白）配體驅(qū)動的 ErbB2-ErbB3 異二聚體是細胞生長和轉(zhuǎn)化方面最普遍和最有效的 復合物。配體激活的 ErbB2-ErbB3 異二聚體信號傳導效力的基礎在于，這種二聚體能夠非 常有效地發(fā)出信號，既可以通過 Ras-ERK（細胞外信號調(diào)節(jié)激酶）途徑進行增殖，也可以 通過 PI3K-Akt/PKB（磷脂酰肌醇-3激酶-Akt/蛋白激酶-B）途徑進行存活。此外，這種受體二聚體可以逃避下調(diào)機制，從而延長信號傳導時間。 | |
| II. Introduction | |
| Host Cell: | MC-38 |
| Expressed gene: | ERBB3 |
| Stability: | 32 passages |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+5μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mouse |
| Tissue: | Colon |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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Figure 1. Recombinant hERBB3/MC38 stably expressing ERBB3. |
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| V. In VIVO Data | |
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Index |
Content |
|
Animal |
C57BL/6J, 6weeks, female |
|
Implanted Position |
Subcutaneous (right lower flank region ) |
|
Injected Cell Numbers |
1×10^6/0.1ml |
|
Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hERBB3(HER3)/MC38 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.hERBB3(HER3)/MC38 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
