hGPC3/H22
CBPG0079
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產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫
| I. Background | |||
| GPC3 是硫酸肝素（heparan sulfate, HS）蛋白多糖家族的一員，該家族包括六個亞型 （GPC1-6）。GPC3 通過糖基磷脂酰肌醇（GPI）錨連接于細(xì)胞膜上，廣泛表達(dá)于人類胚胎， 屬于分泌型蛋白。GPC3 可通過胰島素樣生長因子、骨有關(guān)形成發(fā)生蛋白、成纖維細(xì)胞生 長因子（FGF）或 Hedge-hog 信號調(diào)節(jié)細(xì)胞形態(tài)形成和生長，與多種細(xì)胞表面受體的相互 作用調(diào)節(jié)其下游信號通路，從而調(diào)節(jié)細(xì)胞的黏附、增殖分化、侵襲遷移等生物學(xué)行為。 GPC3 在成人正常組織中含量極少，GPC3 只低表達(dá)于心臟、肺臟、腎臟和卵巢，極少量表 達(dá)于骨骼肌、胰腺、小腸和結(jié)腸。GPC3 在腫瘤組織中的表達(dá)具有差異性，在肝細(xì)胞瘤 （HCC）、非小細(xì)胞肺癌、睪丸和卵巢卵黃囊瘤、惡性黑色素瘤、卵巢透明細(xì)胞癌 （OCCA）、特殊型胃癌（GC）、ESCC、睪丸生殖細(xì)胞瘤、結(jié)腸癌、腎橫紋肌樣瘤中為 高表達(dá)，然而在卵巢癌、膽管癌、GC 和間皮瘤中表達(dá)下調(diào)。 | |||
| II. Introduction | |||
| Host Cell: | H22 | ||
| Expressed gene: | hGPC3 | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | RPMI-1640+10%FBS+1 μg/ml puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mus musculus | ||
| Tissue: | N/A | ||
| Morphology: | N/A | ||
| Growth Properties: | Suspension and lightly attached cells | ||
| IV. Representative Data | ||
|
Figure 1. Recombinant hGPC3/H22 stably expressing GPC3. |
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| V. In VIVO Data | ||
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Index |
Content |
|
Animal |
BALB/C, 8weeks, female |
|
Implanted Position |
Subcutaneous (right lower flank region ) |
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Injected Cell Numbers |
1×10^6/0.1ml |
|
Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hGPC3/H22 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3. hGPC3/H22 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
