hNectin4/RM1
CBPG0094
| I. Background | |||||||||||
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Nectin 細(xì)胞粘附分子 4 (Nectin4)，是一種 I 型跨膜糖蛋白，屬于 Ig 超家族蛋白中的Nectin 家族。除 Nectin-4 外，還有其他三種 Nectin：Nectin-1、Nectin-2 和 Nectin-3。所有Nectin 都具有相同的結(jié)構(gòu)，由三個(gè)細(xì)胞外免疫球蛋白樣結(jié)構(gòu)域（兩個(gè)免疫球蛋白樣 (Ig-like)C2 型結(jié)構(gòu)域和一個(gè) Ig-like V 型結(jié)構(gòu)域）、一個(gè)跨膜區(qū)和一個(gè)胞內(nèi)結(jié)構(gòu)域組成?？扇苄问降腘ectin-4 蛋白是由金屬蛋白酶 ADAM17/TACE 在細(xì)胞表面進(jìn)行蛋白水解裂解而產(chǎn)生的。 Nectin4 在多種腫瘤細(xì)胞中過(guò)表達(dá)，也能促進(jìn)腫瘤細(xì)胞的生長(zhǎng)和增殖。高表達(dá) Nectin4的實(shí)體腫瘤包括膀胱癌、胰腺癌、三陰性乳腺癌、非小細(xì)胞肺癌、胃癌、食道癌、卵巢癌等多種癌癥。 |
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| II. Introduction | |||||||||||
| Host Cell: | RM1 | ||||||||||
| Expressed gene: | Nectin4 | ||||||||||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||||||||||
| Freeze Medium: | 90% FBS+10% DMSO | ||||||||||
| Culture Medium: | DMEM+10%FBS+2μg/ml Puromycin | ||||||||||
| Mycoplasma Testing: | Negative | ||||||||||
| Storage: | Liquid nitrogen | ||||||||||
| III. Description of Host Cell Line | |||||||||||
| Organism: | Mus musculus, mouse | ||||||||||
| Tissue: | Prostate | ||||||||||
| Morphology: | fibroblast-like | ||||||||||
| Growth Properties: | Adherent | ||||||||||
| IV. Representative Data | |||||||||||
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Figure 1. Recombinant hNectin4/RM-1 stably expressing Nectin4. |
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| V. In VIVO Data | |||||||||||
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1. Weight Change
Figure 2. hNectin4/RM1 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.hNectin4/RM1 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
