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HCT116 MTAP-/-cell

CBP75002

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I. Background
MTAP (Methylthioadenosine Phosphorylase) is a Protein Coding gene. Diseases associated with MTAP include Diaphyseal Medullary Stenosis With Malignant Fibrous Histiocytoma and Bone Sarcoma. Among its related pathways are Cytokine Signaling in Immune system and Interleukin-12 family signaling. Gene Ontology (GO) annotations related to this gene include 1,4-alpha-oligoglucan phosphorylase activity and S-methyl-5-thioadenosine phosphorylase activity.
 
II. Description
HCT116 MTAP -/- cells have been homozygously knocked out of MTAP gene.

 

III. Introduction

Host Cell: HCT116
Knockout
gene:
MTAP,Homo sapiens (human)
Stability: 32 passages
Synonym(s): methylthioadenosine phosphorylase,BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: McCoy’s 5A+10%FBS
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Colon
Disease: Colorectal Carcinoma
Morphology: Epithelial-like small cells growing as monolayer
Growth Properties: Adherent
 
V. Representative Data

1. WB of HCT116 MTAP-/-cell

Figure 1. WB of MTAP expression.
Lane 1: HCT116   20ug
Lane 2: HCT116 MTAP -/-   20ug

2. Sanger of HCT116 MTAP-/-cell

Figure 2. Sanger sequencing of MTAP: p.M140Rfs*6.

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of HCT116 MTAP KO cells.

Figure 4. CTG Proliferation Assay of HCT116 Parental cells.

 

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