CX-1（人結腸癌細胞）
CBP61230
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| I. General information | |
| Cell Name: | CX-1 |
| Cell type: | Colon adenocarcinoma (derivative of HT-29) |
| Species: | Homo sapiens，human |
| Description: | Established from the tumor tissue of a 44-year-old woman with well-differentiated colon adenocarcinoma in 1984; cell line is a subclone of cell line HT-29 (DSM ACC 299) |
| Morphology: | Small circular cells, adherent, grow to large colonies in which the single cells are no longer discernible |
| Mycoplasma: | Negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | Cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - |
| Culture Medium: |
DMEM+10%FBS CX-1完全培養(yǎng)基， #CBP61230M |
| Cytogenetics: | Human hypertriploid karyotype with 14% polyploidy - 72(65-74)<3n>XX, -X, +4, +7, -8, -8, -9, +11, +11, -13, +15, +17, -18, +2mar, der(4)t(1;4)(p11;p15)t(1;?) (p32-36;?), del(4)(q32), del(7)(p21), i(13q), add(15)(p11), del(17)(p12), add(21)(p11) - two small markers present - carries 3 markers similar to those present in HT-29 - rearrangements of chs 1, 7, 17 typical of colon carcinoma |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
