NCI-H889（人小細(xì)胞肺癌細(xì)胞）
CBP60147
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| I. General information | |
| Synonyms: | NCI-H889 |
| Background: | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis taken from a patient prior to therapy. |
| Species: | Homo sapiens, human |
| Tissue: | lung; derived from metastatic site: lymph node |
| Disease: | stage E, carcinoma; classic small cell lung cancer |
| Gender: | female, 69 years, Caucasian |
| Morphology: | epithelial |
| Growth Mode: | clusters in suspension |
| DNA Profile: | Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 8 D5S818: 12 D7S820: 8,9 THO1: 8,9.3 TPOX: 8,9 vWA: 17 Our Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+20%FBS NCI-H889完全培養(yǎng)基，# CBP60147M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | The cells express reduced levels of p53 mRNA compared to levels found in normal lung. There is a point mutation, a G to C transition (TGC to TCC), which results in a substitution of a serine for cysteine at codon 242. The cells carry the K-ras 12 mutation. For more information, please contact us (4008-750-250). |
